<?xml version="1.0"?>
<mipe xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
 xsi:noNamespaceSchemaLocation="http://mipe.sourceforge.net/mipe.xsd">
  <version>1.0</version>         <!--#=exactly 1; MIPE version-->
  <pcr id="">                    <!--#=0 or more; actual PCR-->
    <id></id>                    <!--#=exactly 1; ID of PCR product-->
    <modified></modified>        <!--#=1 or more; dates on which PCR record was changed (e.g. 20040503)-->
    <project></project>          <!--#=0 or more; project-->
    <researcher></researcher>    <!--#=1 or more; person(s) changing this PCR-->
    <species></species>          <!--#=1 or more; species on which PCR will run-->
    <design>                     <!--#=exactly 1; stuff concerning PCR primer design-->
      <source>                   <!--#=exactly 1; sequence the primers were designed on-->
	<accession></accession>  <!--#=Exactly 1 from seq, accession or file!!!; accession number-->
	<file></file>            <!--#=Exactly 1 from seq, accession or file!!!; name of file containing sequence-->
        <seq></seq>              <!--#=Exactly 1 from seq, accession or file!!!; actual sequence-->
	<name></name>            <!--#=0 or more; name of source sequence-->
	<species></species>      <!--#=0 or 1; species of source sequence-->
	<type></type>            <!--#=0 or 1; type of sequence (e.g. genomic/mRNA)-->
	<remark></remark>        <!--#=0 or more; remark about source sequence-->
      </source>
      <range></range>            <!--#=0 or 1; e.g. 99-999; pos within source, i.e. the part of the source sequence that is covered in the PCR-->
      <seq></seq>                <!--#=0 or 1; sequence of PCR product including primers-->
      <primer1>                  <!--#=0 or 1; fw PCR primer-->
        <oligo></oligo>          <!--#=0 or 1; oligo number-->
	<seq></seq>              <!--#=0 or 1; sequence-->
	<tm></tm>                <!--#=0 or 1; melting temperature-->
	<remark></remark>        <!--#=0 or more; remark about fw primer-->
      </primer1>
      <primer2>                  <!--#=0 or 1; rev primer-->
        <oligo></oligo>          <!--#=0 or 1; oligo number-->
	<seq></seq>              <!--#=0 or 1; sequence-->
	<tm></tm>                <!--#=0 or 1; melting temperature-->
	<remark></remark>        <!--#=0 or more; remark about rev primer-->
      </primer2>                 
      <profile>
        <name></name>
        <predenaturation>
          <temp></temp>
          <time></time>
        </predenaturation>
        <cycle>
          <number></number>
          <denaturation>
            <temp></temp>
            <time></time>
          </denaturation>
          <annealing>
            <temp></temp>
            <time></time>
          </annealing>
          <elongation>
            <temp></temp>
            <time></time>
          </elongation>
        </cycle>
        <postelongation>
          <temp></temp>
          <time></time>
        </postelongation>
      </profile>
      <remark></remark>          <!--#=0 or more; remark about PCR design-->
    </design>
    <use>                        <!--#=0 or 1; stuff concerning the actual running of the PCR (_after_ design)-->
      <seq></seq>                <!--#=exactly 1; consensus sequence _after_ running the PCR (may differ from seq in design!!)-->
      <revcomp></revcomp>        <!--#=exactly 1; is consensus sequence reverse complement of sequence in design? (if so: 1; else: 0)-->
      <snp>                      <!--#=0 or more; SNPs identified in PCR product-->
        <id></id>                <!--#=exactly 1; SNP id-->
	<pos></pos>              <!--#=exactly 1; position of SNP in use sequence-->
        <pos_design></pos_design><!--#=0 or 1; position of SNP in design sequence-->
	<pos_source></pos_source><!--#=0 or 1; position of SNP in source sequence-->
	<amb></amb>              <!--#=0 or 1; ambiguity code-->
	<rank></rank>            <!--#=0 or 1; if using polyphred for SNP detection: SNP rank (0-6)-->
	<assay>                    <!--#=0 or more; genotyping assay-->
          <type></type>          <!--SBE or RFLP-->
	  <id></id>              <!--#=exactly 1; SBE ID-->
          <enzyme></enzyme>      <!--In case of RFLP: enzyme-->
          <oligo></oligo>        <!--#=0 or 1; SBE oligo number-->
	  <specific></specific>  <!--#=0 or 1; specific sequence of primer-->
	  <tail></tail>          <!--#=0 or 1; tail sequence of primer-->
	  <strand></strand>      <!--#=0 or 1; strand on which SBE primer was designed (relative to use)-->
	  <remark></remark>      <!--#=0 or more; remark about SBE primer-->
	</assay>
	<remark></remark>        <!--#=0 or more; remark about SNP-->
      </snp>
      <sample>                   <!--#=0 or more; samples on which the PCR was run-->
        <id></id>                <!--#=exactly 1; id of sample-->
	<file></file>            <!--#=0 or 1; name of .ab1-file-->
	<genotype>               <!--#=0 or more; for certain/all SNPs (see below): genotype for this sample-->
	  <snp_id></snp_id>      <!--#=exactly 1; must be SNP ID from within this PCR record-->
	  <amb></amb>            <!--#=exactly 1; ambiguity code for this SNP in this sample-->
	  <remark></remark>      <!--#=0 or more; remark about genotype-->
	</genotype>
	<remark></remark>        <!--#=0 or more; remark about sample-->
      </sample>
      <remark></remark>          <!--#=0 or more; remark about use-->
    </use>
    <remark></remark>            <!--#=0 or more; remark about PCR-->
  </pcr>
  <remark></remark>              <!--#=0 or more; remark about MIPE file-->
</mipe>                                            
